To assay for GCRs, fork arrest was induced at RTS1 by inducing Rtf1 (TER2/3 arrest is constitutive) and genomic DNA was analysed by Southern blot. We predicted no GCRs in strains with a single RTS1 ...
Eukaryotic cells respond to nutrients with a variety of signaling pathways, many of which involve activation of protein kinases. Up to now, most attention in studying these pathways has been focused ...